KN-93 hydrochloride Supplier It is important to emphasise th

It is important to emphasise that the present study reports for the first time the isolation and characterisation of myricetin-3-O-(2″-O-galloyl)-α-l-rhamnopyranoside and quercetin-3-O-(2″-O-galloyl)-α-l-rhamnopyranoside in M. pigra L. and the sub-family Mimosoideea. Our result agreed with the findings of [15], which reported the presence of some other acylated flavonol glycosides in M. pigra L. It also further confirmed the evolutionary advancement of M. pigra L. over other Mimosa species [15], since M. pigra is the only Mimosa species so far identified to biosyntheses acylated flavonoids.
Conflict of interest
Acknowledgements Mr Okonkwo, Chinedu J. is grateful to the Nigeria Tertiary Education Trust Fund (TET fund) for the award of a PhD Scholars Grant under the Academic Staff Training and Development (AST&D) Programme. The research was sponsored by the Heinrich-Heine University, Düsseldorf, Germany, under the The World Academy of Science-German Research Foundation (TWASDFG) Postdoctoral Cooperation Research Visit Fellowship with a grant number 3240278143. The authors are greatly indebted to Dr Festus B. C. Okoye of the Department of Pharmaceutical and Medicinal Chemistry, Nnamdi Azikiwe University, Awka, Nigeria, for his immense assistance in the interpretation of the NMR data.
Introduction The World Health Organization estimates that 80% of the world\'s KN-93 hydrochloride Supplier utilizes traditional medicines for primary health care [1]. There is an increasing demand in international market for medicinal plants, which are used for both herbal medicine and as pharmaceutical products. The natural medicines being more safer than synthetic drugs, have gained popularity in recent years, leading to a tremendous growth of phyto-pharmaceutical use. However, herbal medicines can be potentially toxic to human health and sometimes may cause unknown effects. The genus Stapelia belongs to family Asclepiadaceae (Milk Weed Family) [2], and is growing in tropical and southern Africa. Most species are succulent characterized by the presence of a milk juice which is used in some species as an effective arrow poison for fish [3,4]. They are succulent plants, cacti-like. Stapelia species have various uses in medicine as anti tumor, anti-inflammatory, and antiarthritic [2,5]. Stapelia hirsuta L. is a member of this family and is known as dwarf toad plant [6]. Macro and micro-morphological study of the different organs of the plant, isolation of a novel alkaloid from the chloroform fraction of the alcoholic extract, as well as four phenethyl amine alkaloids: N-acetyl hordenine (a new natural compound), hordenine, candicine and hordenine-1-O-β-d-glucoside, in addition to luteolin-7-O-β-d-glucopyranoside have been isolated from n-butanol fraction by the authors [7–9]. This study was aimed to investigate the lipid profile of the selected plant, in addition to, certain bioactivities of the different fractions obtained from total alcohol extract which warrant pharmacological importance of Stapelia hirsuta L.
Material and methods
Results and discussion Four main compounds were isolated from the n-hexane fraction identified as: β-amyrin (1), lupeol (2), α-amyrin (3) and β-sitosterol (4). α-Amyrin was the major compound. The compounds were identified by direct comparison with authentic samples (mixed mp, co-chromatography and mass spectroscopy) as well as by comparison with the published data [22,23]. From Table 1 we can conclude that: the percentage of the identified hydrocarbon, sterols and triterpenes are ≈20.9%, ≈22.6% and ≈51.2%, respectively. The major hydrocarbon is squalene (5%). The β-sitosterol is the major identified sterol (13.9%). The identified triterpenes were; α-amyrin (47.2%) and β-amyrin (4.0%). These results are in accordance with those of the phytochemical study of the n-hexane fraction, which revealed that the α-amyrin as the major compound then the β-sitosterol and finally the β-amyrin.