• YM-155 Hydrochloride: From Signal to Cell Killing

  2026-09-21

  YM-155 hydrochloride is a selective survivin inhibitor whose effects should be interpreted through both growth and cell-death measurements. This guide applies a response-phenotyping framework to assay design, model selection, and translational interpretation.

• Z-VAD-FMK: Reliable Apoptosis Assays

  2026-09-20

  Learn how Z-VAD-FMK (SKU A1902) can clarify caspase-dependent cell death, improve interpretation of viability and proliferation assays, and support better-controlled apoptosis workflows. This scenario-based guide covers assay design, solvent handling, data interpretation, and practical product selection.

• T0070907: Practical PPARγ Assay Design

  2026-09-19

  Learn how T0070907 (SKU A4301) can clarify PPARγ-dependent adipogenesis, viability, and cancer cell-cycle experiments. This scenario-based guide covers mechanism, formulation, protocol controls, interpretation limits, and practical reagent selection.

• V5 Epitope Tag Peptide: Workflow & Troubleshooting

  2026-09-18

  Turn V5-tagged constructs into dependable readouts for Western blotting, immunoprecipitation, localization, and antibody specificity testing. This workflow pairs the high-purity GKPIPNPLLGLDST peptide with kinetic insights from single-molecule antibody screening to improve controls, interpretation, and assay selection.

• Alpha-Ketoglutarate in Tumor Metabolism Workflows

  2026-09-18

  Use alpha-ketoglutarate as a controlled metabolic perturbation to connect TCA-cycle flux, nitrogen handling, enzyme activity, and macrophage signaling. This workflow translates a cholangiocarcinoma mechanism into practical assay design, comparison experiments, and troubleshooting strategies.

• Toxoplasma GRA24, TIMP1, and Brain Barrier Passage

  2026-09-17

  Afanaseva and Barragan show that live Toxoplasma gondii tachyzoites induce host TIMP1 through the parasite effector GRA24 and host p38 MAPK signaling. Rather than simply damaging endothelial barriers, this response appears to support parasite transmigration while preserving monolayer polarization and overall barrier integrity.

• ROS-Responsive Lipid Nanoparticles for Mutant RAS

  2026-09-17

  Cai and colleagues developed a combinatorial library of thioketal-containing, ROS-degradable lipids and identified BAmP-TK-12 as a tumor-selective mRNA delivery material. Its delivery of DUF5-encoding mRNA enabled degradation of mutant RAS and produced stronger antitumor activity than a small-molecule RAS inhibitor in the reported models.

• AG-126: ERK Signaling in Striatal Circuit Studies

  2026-09-16

  AG-126 (Tyrphostin AG-126) offers a focused pharmacological test of ERK1/2 involvement in inflammatory and striatal circuit phenotypes. This article connects its validated biochemical and inflammation evidence with the NLGN1–D2-MSN study while defining the limits of cross-domain interpretation.

• QSHXO in MASLD: Autophagy and Ferroptosis

  2026-09-16

  The reference study shows that Qushi Huoxue ointment improves steatosis and inflammatory liver injury in a mouse model of metabolic associated steatotic liver disease, with evidence for enhanced autophagy and reduced ferroptosis. Its integrated use of serum LC-MS/MS, network pharmacology, molecular validation, and electron microscopy provides a useful framework for dissecting multi-pathway interventions while leaving causal and clinical validation for future work.

• A-1331852 for BCL-XL Apoptosis Assays

  2026-09-15

  A-1331852 is a selective BCL-XL inhibitor for testing mitochondrial apoptosis, BCL-XL–BIM complex disruption, and dependency in cancer models. This practical guide connects dose-response design with senescence-focused experiments while separating established evidence from testable research hypotheses.

• CTOP: μ-Opioid Receptor Antagonist Guide

  2026-09-15

  CTOP is a selective μ-opioid receptor antagonist peptide for receptor-function, signaling, and pain-mechanism studies. It provides a pharmacological way to test whether an opioid response depends on μ-opioid receptor activation, while the 2024 Neuron study supplies circuit-level context for mechanical hypersensitivity and tolerance.

• Maraviroc: From HIV Entry to CCR5 Translation

  2026-09-14

  Maraviroc, also known as UK-427857, is more than a tool for blocking R5-tropic HIV-1 entry. Its ability to perturb CCR5-dependent signaling creates a translational framework for studying viral tropism, extracellular-vesicle biology, neuroinflammation, and disease-specific immune circuits. This article connects mechanistic evidence with practical guidance for designing more interpretable CCR5 studies.

• HyperScribe T7 High Yield Cy5 RNA Labeling Kit

  2026-09-14

  Build sensitive fluorescent RNA probes for in situ hybridization, Northern blot hybridization, and RNA-delivery studies with an adjustable Cy5-UTP substitution strategy. The workflow emphasizes signal-to-yield balance, application-specific controls, and troubleshooting that separates poor transcription from weak detection.

• ARCA EGFP mRNA for Transfection Controls

  2026-09-13

  Use ARCA EGFP mRNA as a rapid, direct fluorescence control for separating delivery failure from weak mammalian cell gene expression. Its capped, polyadenylated design also supports practical comparisons between lipid transfection, in situ electroporation, and emerging delivery workflows.

• ZK53: Human Mitochondrial ClpP Workflows

  2026-09-12

  ZK53 is a selective human mitochondrial serine protease ClpP activator for connecting mitochondrial proteostasis with cancer-cell stress responses. Its orthogonal biochemical assays, cell-based mitochondrial readouts, and reported lung and colorectal cancer applications support a practical workflow from target validation to translational modeling.

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