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Specifications Table
Data
Experimental design, materials and methods
Acknowledgements T.S.S. is grateful to Nadathur Estates for their support of all the breast cancer research activities at SJRI since 2008. We thank the Department of Biotechnology for the DBT-BBI grant (No: BT/PR13926/MED/31/97/2010) to T.S.S. and the University Grants Commission for awarding senior research fellowship (F.2-6/2013(SA-1)) to M.G.N.
Data Fig. 1 shows that the macroscopic pancreatic area of the BALB/c and C57BL/6 mice was not significantly different. The data related to the area and diameter of the pancreatic islets in the C57BL/6 mice were significantly lower than those of BALB/c mice (Fig. 2A–C). In contrast, the circularity of pancreatic islets did not significantly differ between the BALB/c and C57BL/6 mice (Fig. 2D). Fig. 3 shows that the average density of pancreatic islets was significantly higher in the C57BL/6 mice compared to BALB/c mice.
Experimental design, materials and methods
Acknowledgments This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). We thank João Batista Pereira for technical assistance in histology procedures.
Specifications Table
Value of the data
Data The autophagy was increased by 2-DG or rapamycin treatment and a subsequent decrease in insulin production (Figs. 1 and 2). Immunofluorescence staining data showed that 2-DG or rapamycin increased LC3-positive granules or puncta were co-localized with the increased Lamp2, indicating that autophagosomes increase under conditions of 2-DG or rapamycin (Fig. 3). Subsequently, an increase in intracellular insulin was measured in INS-1E insulinoma hematoxylin following treatment with the autophagy inhibitors, bafilomycin A1 and/or 3-methyladenine, in the presence or absence of 2-DG (Fig. 4A and B) or rapamycin (Fig. 4C and D).
Experimental design, materials and methods
Acknowledgment This research was supported by the Leading Foreign Research Institute Recruitment Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2010-00757).
Data Here we provide histological data of tissue sections from the limb skeletal tissues of larval (6.5cm) and adult (25cm) Ambystoma mexicanum (Fig. 1). The images exhibit the morphological characteristics of the limb skeletal tissue in the aging axolotl (6.5cm compared to 25cm long animals), and the purity of the skeletal tissues that have been harvested by manual dissection.
Experimental design, materials and methods
Acknowledgments The authors would like to thank the National Institute of Health through its support of the Ambystoma Genetic Stock Center at the University of Kentucky, Lexington, KY. Catherine McCusker was supported by a Postdoctoral Fellowship, PF-12–145–01-DDC, from the American Cancer Society. Additional support was provided by the US Army Research Office Multidisciplinary University Research Initiative (MURI) (TUL 589-09/10). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Data Fig. 1 contains western blot data of p-Akt/Akt, p-mTOR/mTOR, p-p70s6k/p70s6K, and p-4EBP-1/4EBP-1 in young and aged mice. Fig. 2 contains real time PCR data of FoxO1, FoxO3, MuRF-1, and Atrogin-1 in young and aged mice. Figs. 3 and 4 contain western blot data of Akt, mTOR, p70s6K, and 4EBP-1 at the onset of skeletal muscle regeneration with or without leucine supplementation in young and aged mice, respectively.
Experimental design, materials and methods
Funding This work was supported by the Claude Pepper Older Americans Independence Center (P30 AG028718).
Acknowledgments
Data These data mainly focus on describing intracellular calcium concentration ([Ca2+]i) measured in Wild Type (WT) and mdx cardiomyocytes in two different conditions: at rest and during the application of an axial stretch. Calcium measurement was performed using the Ca2+ dye fluo-8 and during a perfusion protocol starting initially with a calcium free Tyrode solution followed by the perfusion of 1.8mM Ca2+ Tyrode solution. In these conditions, [Ca2+]i kinetics are described (Fig. 1A) and amplitudes are compared in the presence of SACs inhibitors (Fig. 1B). The effect of TRPV2 inhibitors (Fig. 2) and Probenecid (Fig. 3) is also described.