• br DNA fingerprinting analysis The target regions

  2018-10-20

  

  DNA fingerprinting analysis The target regions of genomic DNA were amplified by Polymerase chain reaction (PCR). The PCR was carried out using a Thermocycler type PTC100 (MJ Research Inc) for 35cycles and each cycle contained 96°C for 30s, 56°C for 30s and 72°C for 30s. The following primer seque

• calculate the molarity of a solution br Materials and method

  2018-10-20

  

  Materials and methods Statistical analysis Data are presented as mean±SEM. Differences between groups were detected for statistical significance using the unpaired Student\'s t-test. P Results Discussion We have previously postulated that Hopx might label NSCs in the dentate gyrus based u

• Using a cell state by cell state logistic regression

  2018-10-20

  

  Using a cell-state-by-cell-state logistic regression-based approach (similar in spirit to a one way analysis-of-variance with post hoc analysis), we identified putative core elements of cell-specific transcription for 17 cell states representing nine unique purified human cell types from different g

• br Methods br Results br Discussion

  2018-10-20

  

  Methods Results Discussion Our method for osteoprogenitor cell culture is similar to protocols for mice (Meirelles and Nardi, 2003; Soleimani and Nadri, 2009; Nardi and Camassola, 2011; Abdelmagid et al., 2014; Fig. 1). Marrow and cortex-derived cell populations of bats and mice shared simi

• br Results Relying on the high conservation of

  2018-10-20

  

  Results Relying on the high conservation of vertebrate TBX3 proteins, we used human TBX3 in murine ESCs due to its specific traceability. We inserted human TBX3 cDNA into the pEF-DEST51 vector (Invitrogen) for EF1α-promoter-based stable and constitutive overexpression in ESCs. Out of 20 independe

• In human development OPCs are characterized by

  2018-10-20

  

  In human development, OPCs are characterized by PDGFRα and NG2 expression, followed by expression of O4 (Jakovcevski et al., 2009). Under our culture conditions, by day 75, most of the O4+ cells had lost PDGFRα, but retained NG2 expression. At this stage, we did not observe any residual pluripotent

• br Experimental Procedures iNSCs were generated

  2018-10-20

  

  Experimental Procedures iNSCs were generated, cultured, and transplanted as described previously (Han et al., 2012). In brief, a total of 2.25 × 105 GFP-labeled 4F (Brn4, Sox2, Klf4, and c-Myc) iNSCs that were kept under standard iNSC medium conditions were transplanted into the cortex and hilus

• calmodulin dependent protein kinase Our objective was to det

  2018-10-20

  

  Our objective was to determine how the culturing of CD34+ calmodulin dependent protein kinase under conditions that influence ARNT complex formation might affect the expansion of HSPCs. We hypothesized that the use of culture conditions that stabilize HIF-1α and antagonize the AhR protein would pro

• The following are the supplementary data related

  2018-10-20

  

  The following are the supplementary data related to this article. Conflict of interest Introduction Mammalian eyes contain retinal stem cells (RSCs) that are capable of proliferating and differentiating into all of the cell types of the neural retina as well as the retinal pigmented epitheliu

• Chemotherapy is a standard treatment for

  2018-10-20

  

  Chemotherapy is a standard treatment for patients with advanced disease, including nonresectable, recurrent, or metastatic tumors. Due to chemoresistance, the majority of patients die within three years (Papaspyrou et al., 2011). Indeed, cisplatin and other platinum-based therapies do not present cl

• IOX2 There has been controversy regarding whether subsets

  2018-10-20

  

  There has been controversy regarding whether subsets of premigratory neural crest IOX2 are predetermined to form distinct cell types or multipotent and capable of forming numerous derivatives. Recently, this has been elegantly put to rest by single cell lineage analysis using Confetti transgenic mi

• calcitonin receptor EWS FLI a widely recognized fusion oncog

  2018-10-20

  

  EWS-FLI1, a widely recognized fusion oncogene for Ewing sarcomas, is generated by the chromosomal translocation of t(11;22) (q24;q12), which consists of the N-terminal transactivator domain of the EWS gene and the C-terminal ETS DNA binding domain of the FLI1 gene. The resulting EWS-FLI1 fusion prot

• To address this issue we examined

  2018-10-20

  

  To address this issue, we examined striatal neurons innervated by nigral DA neurons using the plant lectin wheat-germ agglutinin (WGA). WGA can be efficiently transferred between neurons via synapses and has been already used as a transsynaptic tracer (Fabian and Coulter, 1985; Broadwell and Balin,

• br Experimental Procedures br Author Contributions br Acknow

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments We are grateful for the support from ES/iPSC core facility, Lund University. This work was funded by grants from: The Swedish Research Council, Swedish Cancer Society, Swedish Children\'s Cancer Society, AFA Insurance (Sweden), Lu

• The experimental design of this model provides

  2018-10-20

  

  The experimental design of this model provides several differences to standard 2D culture: EHTs are anchored between two flexible silicone posts, which generate a preload, and EHTs perform auxotonic contractile work (the physiological form of cardiac contraction) against elastic posts. This results

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