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br Materials and methods br
2018-11-08
Materials and methods Results Discussion Here we establish a molecular signature for unmodified SCDSs that were initiated by individual multipotent SSCs in comparison with SCDSs that were initiated by ll-37 that were not multipotent, based upon their differentiation capacity as determined
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br Materials and methods br Results and discussion
2018-11-08
Materials and methods Results and discussion Acknowledgments Introduction Reprogramming rejuvenates aged somatic cells back into the pluripotent state (Takahashi et al., 2007; Takahashi and Yamanaka, 2006). The developmental plasticity of induced pluripotent stem cells (iPSC) demonstrate
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A plausible interesting observation coming out
2018-11-08
A plausible interesting observation coming out from this study is that DNA methylation imprint at H19 DMR may be more sensitive to loss of TET proteins in undifferentiated ES serine protease inhibitors than they are in differentiated cells, whereas DNA methylation imprint at Peg1 DMR may be more pro
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Recent methods of reprogramming human fibroblasts to iNSCs e
2018-11-08
Recent methods of reprogramming human fibroblasts to iNSCs employed genome-integrating lentiviral or retroviral vectors (Kumar et al., 2012; Ring et al., 2012), which may produce insertional mutations and residual or reactivation of transgenes. We used oriP/EBNA-1 episomal vectors to deliver reprogr
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In order to assess the
2018-11-08
In order to assess the cellular proliferation potential colony forming efficiency was determined. As we decided to apply a low suction vacuum pressure in order to minimize potential discomfort during bone marrow harvest under local anesthesia in the control individuals the achieved mean cell yields
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br Acknowledgements br Resource table br Resource details
2018-11-08
Acknowledgements Resource table Resource details To generate the Saudi KACST induced Pluripotent Stem Cell line No 1 (SKiPSc1) cell line, four reprogramming Yamanaka factors (Oct3/4, Sox2, c-Myc, and Klf4) (Takahashi et al., 2007; Yu et al., 2007) were delivered to HNFFs by Sendai viral inf
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br Materials and methods br Acknowledgments We
2018-11-08
Materials and methods Acknowledgments We would like to thank Dr. Saran Karumbayaram for providing guidance in the culture and expansion of the primate fibroblasts. We would also like to thank Steven Dowdy for depositing the T7-VEE-OKS-iG plasmid (Addgene). This project was funded by NIH grant
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silybin Induction of EndMT results from integration of multi
2018-11-08
Induction of EndMT results from integration of multiple extrinsic inputs within the dynamically shifting cellular environment of cardiogenesis. Owing to the membrane bound presentation of ligands, Notch signaling status is dependent on the organization and distribution of signal-presenting and signa
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To examine the functional role of IGFBP
2018-11-08
To examine the functional role of IGFBP-2 and -3 we conducted the experiments described in Fig. 4 where the effects of IGFBP-2 and -3 were examined either uncomplexed or complexed with IGF1. The data indicated that IGF1 stimulated ALP activity in DPCs in a dose dependant manner and although there we
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It was recently shown that hPSCs can
2018-11-08
It was recently shown that hPSCs can be expanded as cell-only dnmt in serum-free suspension culture irrespective of matrix supplementation (Amit et al., 2011; Olmer et al., 2010; Singh et al., 2010), which is mandatory for conventional surface-attached propagation of hPSCs. In contrast to matrix-at
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Studies from other glandular tissues like mammary gland reve
2018-11-08
Studies from other glandular tissues, like mammary gland, revealed CD24hi/CD29lo- (Shackleton et al., 2006) expressing NVP-TNKS656 as potential stem cells. In mice, we reported that intraglandular transplantation of primary-sphere-derived CD24+/CD29+ cells could ameliorate radiation-induced hyposali
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The Xenopus homolog of Bucky
2018-11-08
The Xenopus homolog of Bucky ball is known as XVelo (Vegetally localized −1), first identified based on the vegetal pole localization of XVelo RNA (Claussen and Pieler, 2004). A recent study identified XVelo as a Balbiani body enriched protein, and identified prion-like sequences within the amino te
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Since the donor hESC derived cells are allografts there
2018-11-08
Since the donor hESC-derived order Alvespimycin are allografts, there is the possibility of graft rejection after transplantation. Previous allogeneic RPE cell transplantations in dry AMD resulted in graft rejections in some cases, although it started later in the treatment course and was seen less
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br Introduction The adult prostate gland contains a simple c
2018-11-08
Introduction The adult prostate gland contains a simple columnar epithelium composed of luminal secretory and underlying basal PCP inhibitor with a scarce neuroendocrine cell component. These epithelial cells are derived from a rare, relatively quiescent stem cell population that maintains gland
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Although our results suggest that Ronin
2018-11-08
Although our results suggest that Ronin directly impacts DNA repair through the transcriptional regulation of DNA repair genes, we further sought to determine how much the canonical pluripotency factors might contribute to the transcriptional regulation of DNA repair genes. Oct4 and Sox2 are known t
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