br Acknowledgements br Resource table br Resource details

Acknowledgements
Resource table
Resource details To generate the Saudi KACST induced Pluripotent Stem Cell line No 1 (SKiPSc1) cell line, four reprogramming Yamanaka factors (Oct3/4, Sox2, c-Myc, and Klf4) (Takahashi et al., 2007; Yu et al., 2007) were delivered to HNFFs by Sendai viral infection (Life Technologies, Invitrogen) (Ban et al., 2011). SKiPSc1 colonies exhibited a characteristic round shape morphology with small, tightly packed buy Solamargine with a high nucleus/cytoplasm ratio and prominent nucleoli (Fig. 1A). The Sendai virus is non-integrating and the absence of exogenous reprogramming factors and the SeV vector in the cells was confirmed by PCR analysis (Fig. 1B). Pluripotency was verified by endogenous gene expression of pluripotent stem cell markers Oct4, Sox2, Nanog, Klf4 and c-MYc by PCR (Fig. 1B). The colonies were also positive for alkaline phosphatase activity (Fig. 1C). Furthermore, pluripotent surface marker expression of Tra-1-60, Oct4 and SOX2 was confirmed by immunostaining (Fig. 1D). Finally, iPSCs single cells were able to generate embryoid bodies when cultured under certain conditions (Fig. 1E).
Materials and methods